Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. It also integrates with the powerful SoftMax Pro Software for. Published Dec 16, 2020 | Updated Dec 16, 2020. 305 reference level saturation. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. 3% in white 96- and 384. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Populate the following info under Description. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. 0 OD. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Important things to consider when purchasing a multimode microplate reader. Silica excitation fiber, glass emission fiber. 00. Create custom fluorescence assays without having to purchase expensive filter sets. Compare specifications and request a quote from leading suppliers. 5 second read per plate. UV Plate 96 Well, Flat Bottom, Cs 50. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Please refer to the Obsolete Microplate Reader Models for more information. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. These microplates have a corner notch for use in standard plate readers. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. 0% ± 0. (one set per assay) to clear 96-well microplates. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. External Dimensions without lid (mm). The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. It has a 340-750nm Spectral Range. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. 96-well plates - Filter plates with 0. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. Reads various kinds of 96-wells plates, and is equipped with shaking function. Unparalleled performance on a personalized platform. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Supersedes 1. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Working in. Supersedes 1. PURPOSE. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. 9000-0756. Selects either 96-well or 384-well plate for a stand-alone read. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. x. HeLa. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Packed 25 plates per…. * Note: Programming the washer with a final aspirate height of 4. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). 500 OD ± 3. The system features 96, 384,. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. Plate chamber design of the SpectraMax category 1 microplate readers. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. BMG Labtech - THERMOstar. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Microplates, cuvettes (via adapter) Detectors. Plate Shaking. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Tristar 3 Multimode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Sample by eye within . Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Mix samples in microplate wells by user-programmable Automix feature. Microplate Reader. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. 313 reference gain check fail. adjust the reader. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. maximum-illumination-channels. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). to each well. 984. 96- and 384-well. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . We would like to show you a description here but the site won’t allow us. In addition, the iD5 reader measures TRF and FP and. Temperature control: 4°C above ambient to 45°C. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. 9000-0756. x. Corning. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Our. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. FlexIII. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. 96-well microplate compatible Well read area range: D4. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. An industry-renowned instrument for monitoring GPCRs and ion channels. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Cross-talk <0. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. Supplier: Molecular Devices. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. 030 fmol Fluorescence Polarization (FP). Suggested plates are black walled. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Automated live cell imaging – cell counting and confluence. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. 2. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. minutes and begin reading microplates with seven mouse clicks. REF (SpectraMax. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Click Add to create the plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. Return to top. 000 to 2. 340 PC384. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Packed 25 plates per sleeve, 2 sleeves per case. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 2 OD at 200 nm; this decreased to 0. Thermomax model provides thermal control and vertical shake, perfect for. B. Four wash/rinse bottles are included and configurable for both 96- and 384. No accessories are needed and SoftMax® Pro Software. 96-, 384-, and 1536-well microplates. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. M5. Dimensions: 18. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. The picked. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. Description. Browse for different fluorescent readers below and compare across different instrument suppliers. No. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. the Molecular Devices SPECTRAmax® 190 and 250. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. No accessories are needed and SoftMax® Pro Software. 6-384 (96- and 384-well with pipetting) 96-well microplates. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. May require LS-50B…. UV absorbance detection. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. • High antibody-binding surface improves the overall performance in. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. 00. 039. Uncontrolled Copy for Training and Reference Purposes Only . They can be read by most microplate readers. Used. Recertification service. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Gemini XPS Reader scans. High tolerance for contaminants. Service parts for these models are not being produced and are no longer in stock. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. UV Plate 96 Well, Flat Bottom, Cs 50. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Endpoint, kinetic, spectrum. Other technologies such as imaging. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. #SpectraMax L Config) Methods. one 96-well sample plate can be controlled from 15–40°C,. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Features. The 6-well plates were incubated at 37ºC for 9 days before picking. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. These robust readers have been placed in labs from Antarctica to. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 000 to 2. 17,000 cells. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. (96- and 384-well with pipetting) 6-384 (96- and 384-well). The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. ₹ 1,80,000 Get Latest Price. tools to expand the boundaries of their research. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5 fg per well to 0. 000 OD ± 1. This can increase throughput and efficiency, thus saving time and space in the lab. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. . ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Bay Gray powder coated metal back plate, column, and base. µl) was added to each well and incubated at 37°C for 1 h. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Microplate optimization and read height optimization were performed for best results. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Loading Buffer was also added to a set of cell-free wells for background controls. These robust readers have been placed in labs from. Select up to 3 products. A = Log 10 (I 0 /I). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Uncontrolled Copy for Training and Reference Purposes Only . com. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 5032338. • Easy and gentle immobilization of biotin. Contact our support team with your questions regarding Molecular Devices products and applications. #3693; n = 4 for each standard). The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . 2. x. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). of 3 wells of a 96-well plate, from 1 of 3 independent experiments. 0, or. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. x. What are the dimensions of the plate? Code. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Lucid Scientific. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. Plate. Published Dec 16, 2020 | Updated Dec 16, 2020. Service Contracts, Preventative Maintenance and. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 7°C and the bottom to 36. #CA961) Methods. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Related Products: 96 Well Plate Reader. Reader Documentation Version No. Click SoftMax Pro Software User Guide. No. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. 304 signal level saturation. x. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The StakMax stacker also allows users to feed plates into the loading area manually. Materials. 5% at 1. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. U-bottom: 30-40 µl. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. 303 unable to cal dark current. 96- and 384-well microplates. A = 2 - log 10 (%T). Fluorescence Microplate Reader Comparison Chart. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Measurements for every well are automatically tracked over time. TNF standard curve. 96-, 384-, and 1536-well microplates. The top- and bottom-reading Gemini EM Microplate. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Controlled by the computer. D. It features a modular design that allows users to add or change detection cartridges according to their needs. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. 384-WELL SBS STERILE MICROPLATE. d. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. We would like to show you a description here but the site won’t allow us. 96/384-well plate formats. Selects either 96-well or 384-well plate for a stand-alone read. QuickSync. Back plate and…. Visit the supplier page or submit a quote to learn more. 1. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Site. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. the use of a reader calibrated with primary NIST and NMI standards. 300 µL of Detergent Compatible Bradford reagent was added to each well. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. and the A1 corner of the plate adapter (Figure 1-2). Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. Turn the instrument around so that the back of the instrument is. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. SoftMax Pro GxP Software version 5. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. With various on-board features and capabilities, the ThermoMax. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. 1. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology.